On 2021/04/01   /   News_en  

foodproof® STEC Screening and Identification LyoKits Receive AOAC® Certification

– Oktober 2020 –

foodproof STEC STEC Screening and Identification LyoKits Receive AOAC Certification

We are pleased to announce that our foodproof STEC Screening LyoKit and STEC Identification LyoKit in combination with our extraction kit, StarPrep Three, have now received AOAC-RI Performance Tested MethodsSM (PTM) Certification (Certificate No. 102004). The rigorous evaluation, which was conducted with raw ground beef and raw beef trim, was found to meet the appropriate standard for its intended use. With only two PCR reactions, the Shiga toxins (stx1, stx2), the adherence factor intimin (eae) and the eight most important STEC serogroups can be detected and individually identified.

The foodproof STEC Screening and Identification LyoKits were validated in comparison to the reference method USDA-FSIS MLG 5C.00. The foodproof STEC method successfully detected STEC in fresh raw ground beef and fresh raw beef trim after 8 and 22 ± 2 h incubation time for 25 g test portions; and after 12 and 22 ± 2 h incubation time for 375 g test portions. Furthermore, when using the mTSB enrichment medium there was no need for additional antibiotics. This rapid workflow with only two PCR tests ultimately leads to cost-savings and opens up good opportunities for the food sector, especially the meat industry, to generate new sales. Finally, the STEC screening and identification method, with its open platform, is AOAC-RI PTM validated on the following cyclers: LightCycler® 480, LightCycler® 96, AriaMx, CFX96 and ABI 7500 fast, demonstrating its compatibility and versatility.

Product Highlights:

  • Cost and time-saving: With only two PCR reactions, the Shiga toxins (stx1, stx2), the adherence factor intimin (eae) and the eight most important STEC serogroups O26, O45, O103, O104, O111, O121, O145 and O157 can be detected and individually identified

  • Approved: AOAC RI PTM validated (#102004):
    • 8 h enrichment for raw ground beef and raw beef trim (25 g sample)
    • 12 h enrichment for raw ground beef and raw beef trim (375 g sample)obe)
    • mTSB without antibiotics saves costs

  • Flexible: Compatible with all relevant food matrices like raw meat, sausages, raw milk cheese, sprouts, or vegetables

  • Specific: Includes detection of stx2f and in accordance with ISO/TS 13136

  • Robust: High background of only eae-positive bacteria does not inhibit stx detection

  • Convenient: Lyophilized PCR reagents
    • Less pipetting, less contamination risk
    • Higher sensitivity
    • Transportation at room temperature and storage at 2 to 8 °C

  • Innovative: New and unique assay design enables sequence specific melting curve analysis, whereby the highest specificity is achieved

  • Time-saving: 35-cycle colony PCR short protocol with Suspension Buffer (order no. S 400 10) and Internal Amplification Control (order no. A 500 23)