Foodborne Pathogens

A pathogen is a synonym for a disease-causing microorganism. Food pathogens represent a special form of microbial pathogens, which are picked up and spread through food.
Below a number of pathogens are listed, for which BIOTECON Diagnostics offers suitable foodproof® DNA extraction and real-time PCR detection kits. Many of the pathogen kits have been certified by recognized institutions such as AOAC-RI, MicroVal or NordVal (see licensing options / validations), and thus independently tested for quality and safety.

foodproof® STEC Identification LyoKit

Shiga toxin-producing Escherichia coli (STEC), also known as enterohemorrhagic E. coli (EHEC), or verocytotoxin- or verotoxin-producing E. coli (VTEC), have been linked with severe complications like the hemolytic-uremic syndrome (HUS) in humans.

Some STEC serotypes are linked to severe complications more often than others:

      • E. coli O157, accounting for 50 to 80 % of human STEC infections
      • The „Big Six“ O26, O45, O103, O111, O121 and O145, responsible for 70-75 % non-O157 infections
      • E. coli O104, that caused a serious outbreak in Germany in 2011

STEC are most commonly transmitted through raw ground beef, raw or inadequately pasteurized milk, sprouts and vegetables. The foodproof® STEC Identification LyoKit can detect and identify the eight most important STEC serotypes O26, O45, O103, O104, O111, O121, O145 and O157 in just one single PCR test. The completely new and unique assay design enables melting curve analysis with sequence specific 5`Nuclease-probes, whereby highest specificity is achieved.
The foodproof® STEC Identification LyoKit is in accordance with ISO/TS 13136 and USDA-FSIS method MLG 5B.

The foodproof STEC Identification LyoKit was validated according to the AOAC RI PTM program (license number 102004). The validation includes an 8 h rapid enrichment for raw meat samples.

BIOTECON Diagnostics Kit

Available Platforms:

LightCycler 480
  • Reactions: 48
  • Product No.: R 602 12
  • Reactions: 48
  • Product No.: R 602 12


  • E. coli (STEC) O-serotypes O26, O45, O103, O104, O111, O121, O145 and O157
  • Multiple categories
    (please enquire)