Foodborne Pathogens

A pathogen is a synonym for a disease-causing microorganism. Food pathogens represent a special form of microbial pathogens, which are picked up and spread through food.
Below a number of pathogens are listed, for which BIOTECON Diagnostics offers suitable foodproof^® DNA extraction and real-time PCR detection kits. Many of the pathogen kits have been certified by recognized institutions such as AOAC-RI, MicroVal or NordVal (see licensing options / validations), and thus independently tested for quality and safety.

• foodproof Aspergillus Detection LyoKit

• foodproof Brucella Detection Kit
• foodproof Campylobacter Detection Kit
• foodproof Campylobacter Quantification Kit
• foodproof Clostridium botulinum Detection LyoKit
• foodproof Clostridium Quantification LyoKit
• foodproof E. coli and Shigella Detection Kit
• foodproof E. coli O157 Detection Kit
• foodproof STEC Screening LyoKit
• foodproof STEC Identification LyoKit
• foodproof Enterobacteriaceae plus Cronobacter Detection Kit
• foodproof Salmonella plus Cronobacter Detection LyoKit
• foodproof Cronobacter Detection LyoKit
• foodproof Enterobacteriaceae plus Salmonella Detection LyoKit
• foodproof Listeria Genus Detection Kit
• foodproof Listeria plus L. monocytogenes Detection LyoKit
• foodproof Listeria Genus Detection LyoKit
• foodproof Listeria monocytogenes Detection Kit
• foodproof Listeria monocytogenes Detection LyoKit
• foodproof Salmonella Detection Kit
• foodproof Salmonella Detection LyoKit
• foodproof Salmonella Enteritidis & Typhimurium Detection LyoKit
• foodproof Salmonella Genus plus Enteritidis & Typhimurium Detection LyoKit
• foodproof Vibrio Detection LyoKit
• foodproof Yersinia enterocolitica plus Yersinia pseudotuberculosis Detection Kit
• microproof Streptococcus pneumoniae Detection Kit
• foodproof SL Staphylococcus aureus Detection Kit
• foodproof SL Bacillus cereus Detection Kit
• foodproof SL Clostridium perfringens Detection Kit

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foodproof^® STEC Identification LyoKit

Shiga toxin-producing Escherichia coli (STEC), also known as enterohemorrhagic E. coli (EHEC), or verocytotoxin- or verotoxin-producing E. coli (VTEC), have been linked with severe complications like the hemolytic-uremic syndrome (HUS) in humans.

Some STEC serotypes are linked to severe complications more often than others:

• E. coli O157, accounting for 50 to 80 % of human STEC infections
• The „Big Six“ O26, O45, O103, O111, O121 and O145, responsible for 70-75 % non-O157 infections
• E. coli O104, that caused a serious outbreak in Germany in 2011

STEC are most commonly transmitted through raw ground beef, raw or inadequately pasteurized milk, sprouts and vegetables. The foodproof^® STEC Identification LyoKit can detect and identify the eight most important STEC serotypes O26, O45, O103, O104, O111, O121, O145 and O157 in just one single PCR test. The completely new and unique assay design enables melting curve analysis with sequence specific 5`Nuclease-probes, whereby highest specificity is achieved.
The foodproof^® STEC Identification LyoKit is in accordance with ISO/TS 13136 and USDA-FSIS method MLG 5B.

The foodproof STEC Identification LyoKit was validated according to the AOAC RI PTM program (license number 102004). The validation includes an 8 h rapid enrichment for raw meat samples.

Available Platforms: LightCycler 480 Reactions: 48 Product No.: R 602 12 5'Nuclease Reactions: 48 Product No.: R 602 12 Specifications: Target E. coli (STEC) O-serotypes O26, O45, O103, O104, O111, O121, O145 and O157 Matrix Multiple categories (please enquire)

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