Viruses

Foodborne viruses can cause various, sometimes serious conditions. Contamination of foods is usually through infected persons or contaminated waste water. Viruses can not reproduce on food, but small amounts of residing viruses may be sufficient to cause infection.
The standard method for virus detection in food is PCR. BIOTECON Diagnostics offers a norovirus real-time PCR kit specifically for the investigation of food.


virusproof SL SARS-CoV-2 Real-time PCR Kit

The virusproof SL SARS-CoV-2 Real-time PCR Kit* is a one-step reverse transcription real-time PCR assay intended for the in vitro qualitative detection of SARS-CoV-2 for the diagnosis of COVID-19. It requires patient RNA samples extracted and purified from upper and lower respiratory specimen swabs (such as nasopharyngeal or oropharyngeal, sputum and bronchoalveolar lavage fluid) obtained from individuals suspected of having respiratory tract infections.

The kit targets the E gene (E) for Betacoronavirus and the SARS-CoV-2 specific RNA dependent RNA polymerase gene (RdRp) gene. The E gene assay is recommended to screen for SARS-CoV-2, SARS-CoV and SARS-related CoVs and the RdRp gene assay is recommended as a specific confirmation of SARS-CoV-2. For the E gene and RdRp, the fluorescence marker FAM is used, and JOE (VIC/HEX) for the internal control.

The assay is based on the WHO & KCDC reference method and it has been confirmed by in silico analysis with all registered SARS-CoV-2 sequences. The assay is intended for use by trained laboratory personnel who are proficient in performing real-time PCR and in vitro diagnostics procedures.

Additionally needed reagents: H2O PCR-grade (12 x 1 ml; Order no. A 500 19).

*Approved “for research use only”, CE-IVD pending. Approval from the Food and Drug Administration (FDA) for an Emergency Use Authorization (EUA) pending. The assays are based on the WHO reference methods, and according to the Charité (Berlin, Germany) protocol for detection of SARS-CoV-2.

BIOTECON Diagnostics Kit

Available Platforms:

RoboPrep 32, KingFisher FlexTM
  • Reactions: 2×50
  • Product No.: Z 700 20
  • Reactions: 2×100
  • Product No.: Z 700 21

Extraction using the Magnetic Preparation Kit VI in combination

LightCycler 480 II, Applied Biosystems® 7500 fast and CFX96
  • Reactions: 2×50
  • Product No.: Z 700 20
  • Reactions: 2×100
  • Product No.: Z 700 21

Specifications:

Target
  • E gene assay (Betacoronavirus)
  • RdRp gene assay (SARS-CoV-2)

Matrix
  • Clinical specimens, including upper and lower respiratory specimens (such as nasopharyngeal or oropharyngeal swabs, sputum and bronchoalveolar lavage fluid) obtained from individuals suspected of respiratory tract infections.